Multivariate Optimization of a Sulfated- β-Cyclodextrin-Modified Capillary Zone Electrophoretic Method for the Separation of Chiral Arylalcohols | Zendy

Yuping Zhang | Zendy; Hyun-Joo Noh | Zendy; SeongHo Choi | Zendy; Jae Jeong Ryoo | Zendy; KwangPill Lee | Zendy; Kazutoku Ohta | Zendy; Chuzo Fujimoto | Zendy; Jiye Jin | Zendy; Toyohide Takeuchi | Zendy

Open Access

Multivariate Optimization of a Sulfated- β-Cyclodextrin-Modified Capillary Zone Electrophoretic Method for the Separation of Chiral Arylalcohols

Author(s) -

Yuping Zhang,

Hyun-Joo Noh,

SeongHo Choi,

Jae Jeong Ryoo,

KwangPill Lee,

Kazutoku Ohta,

Chuzo Fujimoto,

Jiye Jin,

Toyohide Takeuchi

Publication year - 2004

Publication title -

bulletin of the korean chemical society

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.237

H-Index - 59

eISSN - 1229-5949

pISSN - 0253-2964

DOI - 10.5012/bkcs.2004.25.3.377

Subject(s) - capillary electrophoresis , cyclodextrin , resolution (logic) , chemistry , electrophoresis , chromatography , separation (statistics) , phosphate buffered saline , capillary action , phosphate , analytical chemistry (journal) , materials science , organic chemistry , mathematics , computer science , statistics , artificial intelligence , composite material

Chiral separation of aryalcohols such as 1-phenyl-p ropanol, 1-phenyl-2-proanol, and 2-phenyl-1-propanol by capillary electrophoresis has been optimized using th e overlapping resolution mapping (ORM) scheme. Three critical parameters of the electrophoretic media, i.e. phosphate concentration, sulfated β-cyclodextrin (CD) concentration and pH, were chosen for optimization. The working ranges were initially presumed by 7 preexperiments. Further optimization was carried out by another seven experiments within the narrow working ranges. From the final overlapping resolution mapping a ll peak pairs, the area of maximum separations were located. Using the conditions of a point in this area, we found that the target compounds were a baseline separated within 30 min. The maximum separation conditions of arylalcohols were a chiral selector concentration of 5.4%, a phosphate concentration of 28 mM, and a pH of 5.0.

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