Comparison of Ingredients and Antioxidant Activity of the Domestic Regional Wolfiporia extensa

(, , ) . , oxygen 46~48% 38~39% Carbon, 6.05~6.1% Hydrogen, 0.17~0.21% Nitrogen . 3 sulphur . inductively coupled plasma-mass spectrometry(ICP) 11 S 145~149 ppm, Mg 69~72 ppm, P 122~154 ppm , Ca (509.98 ppm) (210.61 ppm) (223.88 ppm) 2~2.35 . GC-MS oleic aid . 50% DPPH ABTS radical FRAP . 10mg/ml , DPPH ABTS $IC_{50}$ (3 mg/mL), (23.03 mg/mL), (4.16 mg/mL) (3.52 mg/mL), (12.17 mg/mL), (7.40 mg/mL) . FRAP $IC_{50}$ (6.59 mg/mL), (19.06 mg/mL), (18.97 mg/mL) . , , . 【This study was conducted for comparison of ingredients, phytochemical compounds and antioxidant activity of Wofiporia extensa cultured in Gangwon-do, Gyeongsang-do, and Jeolla-do. Three contents of Wofiporia extensa were analyzed as oxygen (46~48%), carbon (38~39%), hydrogen (6.05~6.1%) and nitrogen (0.17~0.21%). The mineral contents of 50% ethanol Wofiporia extensa extracts were measured as sulfur (S) 145~149 ppm, Magnesium (Mg) 69~72 ppm, phosphorus (P) 122~154 ppm and calcium (Ca) 210.61~509.98 ppm. Wofiporia extensa from Gyeongsang-do (509.98 ppm) contained a significantly higher quantity of Ca than that from Gangwon-do (210.62 ppm) and Jeolla-do (223.88 ppm). In the gas chromatograph-mass spectrometry (GC-MS) analysis, oleic acid was identified in three 50% ethanol Wofiporia extensa extracts. In the 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2'-azino-bis-3-ethylbenzothiazoline-6-sulphonic acid (ABTS) assay for antioxidant activity, the $IC_{50}$ values of Wofiporia extensa cultured in Gangwon-do, Gyeongsang-do and Jeolla-do were calculated as 2.966 mg/mL, 23.03 mg/mL, and 4.16 mg/mL and 3.521 mg/mL, 12.17 mg/mL, and 7.40 mg/mL. In the ferric reducing antioxidant power (FRAP) assay, the $IC_{50}$ values of Wofiporia extensa cultured in Gangwon-do, Gyeongsang-do, Jeolla-do were 6.585 mg/mL, 19.06 mg/mL, and 18.97 mg/mL, respectively. In summary, Wofiporia extensa cultured in Gangwon-do had stronger antioxidant activity and higher concentration of oleic acid than that of Geyongsang-do and Jeolla-do. However, Wofiporia extensa cultured in Geyongsang-do contained a much higher concentration of Ca than that of Gangwon-do and Jeolla-do.】