Experimental study of recombinant aukaryotic expression vector of human eNOS in ECV304 cells | Zendy

Tong Qiao | Zendy; Changjian Liu | Zendy; Ran Feng | Zendy; Han Li | Zendy; Le Zhang | Zendy; Li Lei | Zendy

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Experimental study of recombinant aukaryotic expression vector of human eNOS in ECV304 cells

Author(s) -

Tong Qiao,

Changjian Liu,

Ran Feng,

Han Li,

Le Zhang,

Li Lei

Publication year - 2006

Publication title -

schweizerische medizinische wochenschrift

Language(s) - English

Resource type - Journals

ISSN - 0036-7672

DOI - 10.4414/smw.2006.10910

Subject(s) - enos , transfection , microbiology and biotechnology , lipofectamine , umbilical vein , complementary dna , nitric oxide synthase , nitric oxide , medicine , biology , recombinant dna , biochemistry , in vitro , gene , vector (molecular biology)

Gene transfer with recombinant non-viral vectors encoding vasodilator proteins, such as endothelial nitric oxide synthase (eNOS), maybe a preferential choice in gene therapy of artery restenosis following angioplasty, stent or anastomosis. However, the transfection rate of a non-viral vector, the harmful effects of eNOS transfection on endothelial cells (EC) and the control release of nitric oxide (NO) have been controversial. We designed the eukaryotic expression vector pcDNA3.1-eNOS to study the regulated expression of eNOS (in the presence of various chemical agents) and to evaluate the exogenous NO effect on EC proliferation in vitro.

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