Electroanalytical determination of reduced-glutathione in biological samples

DOI: 10.4328/JCAM.6155 Received: 07.01.2019 Accepted: 14.01.2019 Published Online: 14.01.2019 Printed: 01.05.2019 J Clin Anal Med 2019;10(3): 394-7 Corresponding Author: Ebru Kuyumcu Savan, Faculty of Pharmacy, Analytical Chemistry Division, İnönü University, Malatya, Turkey. T.: +90 4223411216 F.: +90 4223411217 E-Mail: ebru.savan@inonu.edu.tr ORCID ID: 0000-0002-8851-0907 Abstract Aim: In this study, it was aimed to determine the reduced Glutathione (GSH) at the modified sensor with a simple and reliable electroanalytical method. Material and Method: Cyclic voltammetry and differential pulse voltammetry techniques were used for the determination of reduced-GSH. And the modified sensor was fabricated by dropping single-walled carbon nanotube (SWCNT) dispersion onto the surface of the glassy carbon electrode. Results: The determination of reduced-GSH was accomplished at SWCNT modified sensor. The relationship between the current responses of the sensor and the concentrations of the reduced-GSH (1.0 x 10-8 5.0 x 10-8 M) showed excellent linearity and the detection limit was calculated as 75 nM. Discussion: Determination of trace amounts of reduced-GSH in urine samples was successfully applied at the modified sensor. The results showed that modification of the glassy carbon electrode with SWCNT could provide a new strategy for determining the concentration of GSH in physiological solutions.