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The tomato is a culture that demands high volume of water during irrigation, may limit its yield under water deficit. Therefore, the selection of genotypes tolerant to water deficit can benefit crop management, thus favoring higher productivity. The objective of this study was to evaluate the potential of mannitol osmotic solution as water deficit simulator for selection of tomato genotypes. The experiments were conducted in the laboratory and in the field, in a completely randomized design with four replications. In laboratory evaluated the best concentration of mannitol able to identify two contrasting genotypes (wild accession LA-716, of the species Solanum pennellii and Santa Clara) known to be tolerant and susceptible to water deficit, respectively. The osmotic potential of -0.3 MPa obtained from the use of 22.29 g L-1 mannitol highest efficiency for selection of tomato genotypes. This concentration was used for selection in seven segregating genotypes F2RC1, from interspecific cross (S. pennellii vs. S. lycopersicum) compared to the resistant witness, wild accession LA-716 (S. pennellii) and susceptible (Santa Clara). The UFU80 F2RC1#1 genotype was the best, being statistically higher compared with the susceptible witness to water deficit (Santa Clara). The osmotic potential of -0.3 MPa obtained by mannitol was effective in indirect selection of tomato segregating genotypes tolerant to drought deficit. Déficit hídrico induzido por manitol para seleção de genótipos de tomateiro Water deficit induced by mannitol for selection of tomato genotypes Gabriel Mascarenhas Maciel1* Andrea Farneze Berenguer1 Ernani Clarete da Silva2 Eusímio Felisbino Fraga Junior1 Debora Kelli Rocha1 Jaíne Priscila Rodrigues da Rocha1