Open AccessThe conservative behaviour of fluorescein
Author(s) -
Simon A. Smith,
WA Pretorius
Publication year - 2002
Publication title -
water sa
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.389
H-Index - 59
eISSN - 1816-7950
pISSN - 0378-4738
DOI - 10.4314/wsa.v28i4.4913
Subject(s) - absorbance , fluorescein , molar absorptivity , chemistry , tracer , chromatography , fluorescence , optics , physics , nuclear physics
Failure to account for fluorescein absorbance changes with pH may be responsible for some of the apparent non-conservative behaviour of this easily detectable tracer compound. While it is possible to calculate an accurate absorptivity value for fluorescein at every pH, this calculation is not necessary if the sample pH is increased above pH 9 before measuring the absorbance. Intense sunlight degrades fluorescein quickly but even hot samples are stable if kept in the dark.
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