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Escherichia coli is the most prevalent organism responsible for urinary tract infections (UTIs) in hospital and community sources. The present study was carried out to detect multi drug resistant (MDR) E coli from urine samples and the role of plasmids in drug resistance. One hundred urine samples were collected from the hospital and community within the University of Port Harcourt. Microscopic and chemical examination was carried out on the urine samples. E coli were isolated and antibiotic sensitivity test was carried out on the isolates, the resistant E. coli were cured by acridine orange and further subjected susceptibility testing. Result obtained from the study showed 35% E. coli recovered from community samples and 65% from hospital samples. Antibiotic sensitivity testing before plasmid curing showed high level of resistance to Augmentin (99%), Cefuroxime (92%), Ceftazidime (78%) and Cefixime (71%). The lowest level of resistance was reported in Gentamicin (15%) and Nitrofurantoin (19%). All the isolates were resistant to Augmentin but upon plasmid curing the resistant rate of isolates to eight antibiotics reduced. Our findings showed that Augmentin and Cefuroxime (62 and 31%) were still resistant after the plasmids of the isolates were cured. For hospital and community sources Nitrofurantoin (1; 0%), Ceftazidime (3; 8%), Ciprofloxacin (1%), Gentamicin (10%) and Ofloxacin (10%). Sixty-two (62) percent of the hospital isolates were resistant to three or more antibiotics while 60% of the community isolates were multidrug resistant. Our study thus concludes that plasmids alone are not responsible for the resistance to antibiotics exhibited by E,coli from urine samples. Antibiotics should be produced to target genes that are responsible for resistance to prevent the spread of drug resistant organisms. Keywords : Antibiotics, Escherichia coli , Multidrug resistant, Plasmid

Plasmid Curing in Multi-Drug Resistant Hospital and Community Uropathogenic Escherichia coli