Toxicity evaluation of waste effluent from cassava-processing factory in Lagos state, Nigeria using the Allium cepa assay | Zendy

A.K. Ogunyemi | Zendy; T.A Samuel | Zendy; O.O. Amund | Zendy; M.O. Ilori | Zendy

Open Access

Toxicity evaluation of waste effluent from cassava-processing factory in Lagos state, Nigeria using the <i>Allium cepa</i> assay

Author(s) -

A.K. Ogunyemi,

T.A Samuel,

O.O. Amund,

M.O. Ilori

Publication year - 2017

Publication title -

deleted journal

Language(s) - English

Resource type - Journals

ISSN - 0794-4896

DOI - 10.4314/ijs.v19i2.17

Subject(s) - mitotic index , allium , chemistry , effluent , genotoxicity , horticulture , chromosome aberration , wastewater , botany , biology , biochemistry , mitosis , toxicity , waste management , chromosome , organic chemistry , gene , engineering , microbiology and biotechnology

Mutagenic and genotoxic effects of cassava wastewater (CWW) were investigated by assay of Allium cepa root meristematic cells. The physicochemical parameters of the wastewater samples were also determined. In Allium root growth inhibition test, experimental onion bulbs were cultivated in various concentrations of the CWW and distilled water was used as a negative control. After 72 h, the root tips from the treated bulb were processed for cytological studies by orcein squash technique. The mean lengths of root bundles were obtained and effective concentration (EC) values calculated. The cytotoxic effects on the onion root tips showed strong growth retardation at high concentrations of the effluent with EC value of 5.67%. The mitotic index (MI) 50

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