Etude de la sensibilité aux antibiotiques des mycoplasmes isolés de spermes à Abidjan

Les mycoplasmes sont des bacteries du tractus uro-genital souvent associes a l’infertilite masculine. Au plan therapeutique, des resistances acquises vis-a-vis des antibiotiques ont ete rapportees. Ce travail avait pour objectif de determiner le niveau de sensibilite des mycoplasmes uro-genitaux isoles au cours de la spermoculture. L’etude retrospective, a porte sur 313 mycoplasmes identifies grâce au kit Mycoplasma IST2 (Biomerieux, Ref 42 505). Ce kit permet la quantification differentielle, l’identification de Ureaplasma urealyticum , et Mycoplasma hominis , ainsi que l’etude de la sensibilite a neuf antibiotiques ( josamycine, azithromycine, erythromycine, clarithromycine, pristinamycine, tetracycline, doxycycline, ofloxacine etciprofloxacine ). Ureaplasma urealyticum a ete identifie dans 245 echantillons (78,3%) et M ycoplasma hominis dans 27 (8,6%). La co-infection ( Ureaplasma urealyticum/Mycoplasma hominis ) a ete retrouvee chez 41 patients (13,1%). La josamycine etait active sur l’ensemble des germes avec des sensibilites respectives de 90,2% et 92,6% pour Ureaplasma urealyticum et pour Mycoplasma hominis. L’activite des cyclines sur Ureaplasma urealyticum etait bonne tandis que celle des fluoroquinolones etait mediocre. La pristinamycine etait efficace sur Mycoplasma hominis (70%). L’etude a montre que la josamycine etait active sur l’ensemble des mycoplasmes, les cyclines conservaient une bonne efficacite sur Ureaplasma urealyticum contrairement aux fluoroquinolones. Ces variations de sensibilite justifient l’interet de l’identification des deux especes et l’etude de la sensibilite pour une bonne prise en charge de ces infections.  Mots cles : Mycoplasmes uro-genitaux, sperme, sensibilite, antibiotiques. English Title: Antibiotic susceptibility of urogenital mycoplasma strains isolated from sperms in Abidjan Mycoplasma urogenital tract bacteria that are often associated with male infertility. At the therapeutic level, acquired resistance to antibiotics has been reported. The objective of this work was to determine the sensitivity level of urogenital mycoplasma strains isolated during sperm culture. Retrospective study focused on 313 mycoplasma identified with Mycoplasma IST2 kit (Biomerieux Ref 42 505). This kit allows differential quantification, identification of Ureaplasma urealyticum, and Mycoplasma hominis, as well as sensitivity studies to nine antibiotics ( josamycin, azithromycin, erythromycin, clarithromycin, pristinamycin, tetracycline, doxycycline, ofloxacin and ciprofloxacin ). Ureaplasma urealyticum was identified in 245 samples (78.3%) and Mycoplasma hominis in 27 (8.6%). Co-infection ( Ureaplasma urealyticum/Mycoplasma hominis ) was found in 41 patients (13.1%). Josamycin was active on all germs with sensitivities of 90.2% and 92.6% respectively for Ureaplasma urealyticum and Mycoplasma hominis. Cycline activity on Ureaplasma urealyticum was good while fluoroquinolone activity was poor. Pristinamycin was effective on Mycoplasma hominis (70%). The study showed that josamycin was active on all mycoplasma, cyclins retained good efficacy on Ureaplasma urealyticum unlike fluoroquinolones. These variations in sensitivity justify the interest of identifying the two species and studying the sensitivity for a good management of these infections. Keywords: Urogenital mycoplasma , sperm, suceptibility, antibiotics.