Open AccessBiological hardening and genetic fidelity testing of micro-cloned progeny of Chlorophytum borivilianum Sant . et Fernand .
Author(s) -
Archana Mathur,
Ajay Kumar Mathur,
Priyanka Verma,
Shrawan Yadav,
Moti Lal Gupta,
Mahendra P. Darokar
Publication year - 2008
Publication title -
african journal of biotechnology
Language(s) - English
Resource type - Journals
ISSN - 1684-5315
DOI - 10.4314/ajb.v7i8.58601
Subject(s) - biology , microbial inoculant , rapd , shoot , horticulture , botany , plantlet , genetic diversity , tissue culture , genetics , inoculation , in vitro , population , demography , sociology
The micro-cloned plantlets of Chlorophytum borivilianum registered more than 95% establishment in soil following treatment with various bio-inoculants namely; Glomus aggregatum, Trichoderma harazianum and Piriformospora indica whereas Azospirullum sp. (CIM-azo) and Actinomycetes sp. (CIM-actin) showed only up to 85% plantlet establishment. The un-rooted shoots were also treated with these bio-inoculants, for in vivo root induction and increased survival rate/establishment frequency when transferred to soil. The un-rooted shoots also showed in vivo rooting (50%) when treated with mycorrhiza Glomus aggregatum (VAM) and Trichoderma harazianum. The genetic fidelity testing of micro-cloned, bio-hardened progeny based on a RAPD analysis using 40 random decamer DNA primers indicated a strong uniformity in relation to the parent genotype.
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