A Rat Model of Acute Respiratory Distress Silymarin’s Antiinflamatory and Antioxidant Effect

Objective: In this study, it was\udaimed to evaluate the anti-inflammatory and\udantioxidative effects of Silymarin in rats in\udwhom artificial acute pulmonary damage\udwas provided with caecal ligation-perforation\udmethod.\udMaterial and Method: Forty-six rats were\udrandomized to sham (n=14), control (n=16),\udsilymarin (n=16) groups. Each group had\udearly and late subgroups. Silimarin was\udadministered in the silimarin group and saline\udwas administerd in control and sham groups.\udArtificial acute pulmonary damage associated\udwith sepsis was provided with caecal ligationperforation\udmethod in control and silimarin\udgroups. Rats in the early subgroup Were\udterminated at the end of the 12th hour and\udthreats in the late group were followed-up.\udSerum and bronchoalveolar lavage fluid\ud(BAL) tumor necrosis factor (TNF)-alpha,\udinterleukin (IL)-1beta, and IL-6; lung tissue\udmalondialdehyde (MDA) and glutathione (GSH)\udlevels; lung histopathologic examination;\udand lung wet-to-dry (w/d) weight ratio\udmeasurements were used to compare and\udevaluate the severity of lung injury between the\udgroups.\udResults: Mortality rates for silymarin and control\udgroups were 62.5% and 12.5%, respectively\ud(log-rank p=0.0506). Compared with the\udsilymarin group, the control group exhibited\udsignificantly more severe lung injury, as\udindicated by higher mean values for serum and\udBAL TNF-alpha, IL-1beta and IL-6 (p<0.05 for all\udmeasurements), total lung histopathologic injury\udscore (p=0.001), w/d (p=0.019) and lung-tissue\udMDA (p=0.011) levels. Lung tissue GSH levels\udwere significantly higher in silymarin group than\udcontrol group (p=0.001).\udConclusion: Silymarin reduces the severity of\udsepsis induced-acute lung injury and may also\udimprove survival in a cecal ligation and perforation\udrat model. These beneficial effects of this agent\udare probably due to its inhibitory effects on\udinflammatory process and oxidative injury