In-vitro Investigation of Imidaclopride effects on AChE, LDH and GSH levels in L-929 Fibroblast Cell Line

There are several type of pesticides to control pests and several new type of pesticides come into use that could less toxic compared to old ones. Pesticide induced oxidative stress which is one of the main mechanism of toxicity, mostly focused research area in the last decade. There are several different studies in the literature on whether pesticide exposure induce oxidative stress parameters mediated toxicity... Pesticide induced oxidative stress level depends on biochemical feature of mammalian systems. Imidacloprid is a neonicotinoid pesticide which widely use that considered safe, however it has been reported in different studies that imidacloprid may cause changes oxidative stress parameters. METHODS: Thus, in this study we aimed to investigate dose and time dependent imidaclopride effects on AcHE, LDH and GSH levels in L-929 fibroblast cell line. 1-500 μg imidaclopride dose range effects on acetylcholinesterase, glutathione and lactat dehidrogenase were investigated in this study. RESULTS: LDH levels were significantly increased dose dependently in 250 and 500 ng imidaclopride compared to control group. GSH levels non-significantly decreased dose dependently and GSH levels were decreased in 500 ng imidaclopride group compared to control. There were no significant difference between groups for acetylcholinesterase levels. DISCUSSION AND CONCLUSION: These results indicated that high doses imifoclopride may induce oxidative stress parameters in fibroblast cells.