İntravitreal Bevacizumabın Sıçan Retina Hücrelerinde Apoptozis Üzerine Etkileri

Pur po se: To investigate the apoptotic effects of intravitreal bevacizumab on rat retinal cells.\udMa te ri al and Met hod: Thirty-six male adult Swiss albino rats were randomly divided into two groups. The first group was applied 0.25\udmg bevacizumab in 0.01 ml saline, and the second group received the same amount of saline intravitreally. Each group was divided into 3\udsubgroups. The animals were sacrificed and their globes were enucleated at the 3rd, 24th, and 72nd hours of the experiment. Enucleated eyes\udwere preserved for histological analysis, immunohistochemical analysis of caspase-3, caspase-8, caspase-9, Fas/Fas L, VEGF and VEGF receptors\ud(Flt-1, Flk-1), and TUNEL staining.\udRe sults: Histological evaluation showed no sign of retinal toxicity in both groups. In TUNEL staining, TUNEL(+) cells were\uddetected in all subgroups, but the number of positive cells was relatively lower in bevacizumab treatment group that reached\udstatistically significant level at 24 and 72 hours of treatment (p<0.001). Immunohistochemical analysis revealed that in saline-treated\udsubgroups, immunoreactivity was more pronounced for all apoptosis-inducing proteins compared to bevacizumab-treated group. Also\udimmunoreactivity of VEGF was prominent in saline treated group. For VEGF receptors, staining was only positive for Flt-1 at the 3rd\udhour in the control group.\udDis cus si on: Bevacizumab did not have apoptosis-inducing potential compared to saline solution in short term, which was\uddocumented with TUNEL and immunohistochemical staining. (Turk J Ophthalmol 2013; 43: 39-44