Open AccessExperimental Transmission of Mayaro Virus by Aedes aegypti
Author(s) -
Kanya C. Long,
Sarah A. Ziegler,
Saravanan Thangamani,
Nicole Hausser,
Tadeusz J. Kochel,
Stephen Higgs,
Robert B. Tesh
Publication year - 2011
Publication title -
american journal of tropical medicine and hygiene
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.015
H-Index - 151
eISSN - 1476-1645
pISSN - 0002-9637
DOI - 10.4269/ajtmh.2011.11-0359
Subject(s) - aedes aegypti , virology , biology , chikungunya , vector (molecular biology) , transmission (telecommunications) , virus , outbreak , zika virus , blood meal , titer , aedes albopictus , yellow fever , dengue fever , zoology , larva , ecology , genetics , engineering , gene , electrical engineering , recombinant dna
Outbreaks of Mayaro fever have been associated with a sylvatic cycle of Mayaro virus (MAYV) transmission in South America. To evaluate the potential for a common urban mosquito to transmit MAYV, laboratory vector competence studies were performed with Aedes aegypti from Iquitos, Peru. Oral infection in Ae. aegypti ranged from 0% (0/31) to 84% (31/37), with blood meal virus titers between 3.4 log(10) and 7.3 log(10) plaque-forming units (PFU)/mL. Transmission of MAYV by 70% (21/30) of infected mosquitoes was shown by saliva collection and exposure to suckling mice. Amount of viral RNA in febrile humans, determined by real-time polymerase chain reaction, ranged from 2.7 to 5.3 log(10) PFU equivalents/mL. Oral susceptibility of Ae. aegypti to MAYV at titers encountered in viremic humans may limit opportunities to initiate an urban cycle; however, transmission of MAYV by Ae. aegypti shows the vector competence of this species and suggests potential for urban transmission.
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