Open AccessCharacterization of Novel Leishmania infantum Recombinant Proteins Encoded by Genes from Five Families with Distinct Capacities for Serodiagnosis of Canine and Human Visceral Leishmaniasis
Author(s) -
Geraldo Gileno de Sá Oliveira,
Franklin Barbalho Magalhães,
Márcia Cristina Aquino Teixeira,
Andréa Mendes Pereira,
Cristiane Garboggini Melo de Pinheiro,
Lenita Ramires dos Santos,
Marília B. Nascimento,
Cheila Nataly Galindo Bedor,
Alessandra Lima de Albuquerque,
Washington L. C. dosSantos,
Yara M. Gomes,
Edson Duarte Moreira,
Maria Edileuza Felinto de Brito,
Lain C. Pontes de Carvalho,
Osvaldo P. de Melo Neto
Publication year - 2011
Publication title -
american journal of tropical medicine and hygiene
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.015
H-Index - 151
eISSN - 1476-1645
pISSN - 0002-9637
DOI - 10.4269/ajtmh.2011.11-0102
Subject(s) - leishmania infantum , visceral leishmaniasis , leishmaniasis , recombinant dna , canine leishmaniasis , leishmania , biology , gene , virology , computational biology , genetics , parasite hosting , computer science , world wide web
To expand the available panel of recombinant proteins that can be useful for identifying Leishmania-infected dogs and for diagnosing human visceral leishmaniasis (VL), we selected recombinant antigens from L. infantum, cDNA, and genomic libraries by using pools of serum samples from infected dogs and humans. The selected DNA fragments encoded homologs of a cytoplasmic heat-shock protein 70, a kinesin, a polyubiquitin, and two novel hypothetical proteins. Histidine-tagged recombinant proteins were produced after subcloning these DNA fragments and evaluated by using an enzyme-linked immunosorbent assays with panels of canine and human serum samples. The enzyme-linked immunosorbent assays with different recombinant proteins had different sensitivities (67.4-93.0% and 36.4-97.2%) and specificities (76.1-100% and 90.4-97.3%) when tested with serum samples from Leishmania-infected dogs and human patients with VL. Overall, no single recombinant antigen was sufficient to serodiagnosis all canine or human VL cases.
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