Detection of the Dihydrofolate Reductase–164L Mutation in Plasmodium falciparum Infections from Malawi by Heteroduplex Tracking Assay | Zendy

Jonathan J. Juliano | Zendy; Victor Mwapasa | Zendy; Steven R. Meshnick | Zendy; Paul A Trottman | Zendy

Open Access

Detection of the Dihydrofolate Reductase–164L Mutation in Plasmodium falciparum Infections from Malawi by Heteroduplex Tracking Assay

Author(s) -

Jonathan J. Juliano,

Victor Mwapasa,

Steven R. Meshnick,

Paul A Trottman

Publication year - 2008

Publication title -

american journal of tropical medicine and hygiene

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.015

H-Index - 151

eISSN - 1476-1645

pISSN - 0002-9637

DOI - 10.4269/ajtmh.2008.78.892

Subject(s) - dihydrofolate reductase , heteroduplex , plasmodium falciparum , biology , mutation , polymerase chain reaction , virology , drug resistance , point mutation , genetics , malaria , gene , immunology

Standard polymerase chain reaction methods often cannot detect drug-resistance mutations in Plasmodium falciparum infections if the mutation is present in < or = 20% of the parasites. A heteroduplex tracking assay was developed that can detect dihydrofolate reductase 164-L mutations in variants representing 1% of the parasites in an individual host. Using this assay, we confirmed the presence of the mutation in P. falciparum infections in Malawi.

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