Open AccessDETERMINANTS OF VECTOR SPECIFICITY OF O’NYONG NYONG AND CHIKUNGUNYA VIRUSES IN ANOPHELES AND AEDES MOSQUITOES
Author(s) -
Dana L. Vanlandingham,
Stephen Higgs,
Chao Hong,
Kimberly A. Klingler,
Kate L. McElroy,
M. J. Lehane,
Konstantin A. Tsetsarkin
Publication year - 2006
Publication title -
american journal of tropical medicine and hygiene
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.015
H-Index - 151
eISSN - 1476-1645
pISSN - 0002-9637
DOI - 10.4269/ajtmh.2006.74.663
Subject(s) - chikungunya , biology , virology , anopheles gambiae , vector (molecular biology) , aedes aegypti , virus , alphavirus , togaviridae , culex tritaeniorhynchus , anopheles , yellow fever , aedes , arbovirus , chimera (genetics) , dengue fever , gene , japanese encephalitis , malaria , genetics , larva , encephalitis , recombinant dna , botany , immunology
The alphaviruses o'nyong nyong virus (ONNV) and chikungunya virus (CHIKV) provide a unique system to study the viral genes involved in vector specificity. ONNV infects both anopheline and culicine mosquitoes, whereas CHIKV infects only culicine mosquitoes. In this study, chimeric viruses were constructed that contained genes from both ONNV and CHIKV. These chimeras and previously described full-length infectious clones of ONNV and CHIKV were evaluated in Anopheles gambiae and Aedes aegypti mosquitoes. Virus derived from the infectious clones of ONNV and CHIKV retained the vector specificity of the parental viruses. All six of the chimeras were found to infect Ae. aegypti mosquitoes at high rates but only the chimera containing viral genes encoding all of the structural proteins of ONNV was able to infect An. gambiae mosquitoes. These data indicate that all of the viral structural proteins are necessary for ONNV to infect An. gambiae mosquitoes.
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