Open Accesspfmdr1 GENOTYPING AND IN VIVO MEFLOQUINE RESISTANCE ON THE THAI-MYANMAR BORDER
Author(s) -
Amy Nelson,
Anne Purfield,
Philip McDaniel,
Nichipat Uthaimongkol,
Nillawan Buathong,
Sabaitip Sriwichai,
R. Scott Miller,
Chansuda Wongsrichanalai,
Steven R. Meshnick
Publication year - 2005
Publication title -
american journal of tropical medicine and hygiene
Language(s) - English
Resource type - Journals
eISSN - 1476-1645
pISSN - 0002-9637
DOI - 10.4269/ajtmh.2005.72.586
Subject(s) - mefloquine , plasmodium falciparum , malaria , drug resistance , biology , genotyping , single nucleotide polymorphism , in vivo , artemisinin , pharmacology , virology , genotype , gene , genetics , immunology
Molecular markers have been proposed as a method of monitoring malaria drug resistance and could potentially be used to prolong the life span of antimalarial drugs. Single nucleotide polymorphisms (SNPs) in the Plasmodium falciparum gene pfmdr1 and increased gene copy number have been associated with in vitro drug resistance but have not been well studied in vivo. In a prospective cohort study of malaria patients receiving mefloquine treatment on the Thai-Myanmar border, there was no significant association between either pfmdr1 SNPs or in vitro drug sensitivity and mefloquine resistance in vivo. Increased pfmdr1 gene copy number was significantly associated with recrudescence (relative risk 2.30, 95% CI 1.27-4.15). pfmdr1 gene copy number may be a useful surveillance tool for mefloquine-resistant falciparum malaria in Thailand.
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