POLYMORPHISM OF THE PLASMODIUM FALCIPARUM MULTIDRUG RESISTANCE AND CHLOROQUINE RESISTANCE TRANSPORTER GENES AND IN VITRO SUSCEPTIBILITY TO AMINOQUINOLINES IN ISOLATES FROM THE PERUVIAN AMAZON | Zendy

Maria Cecilia Huaman | Zendy; Norma Roncal | Zendy; Shusuke Nakazawa | Zendy; Ton That Ai Long | Zendy; Lucia Gerena | Zendy; Coralith García | Zendy; Lely Solari | Zendy; Alan J. Magill | Zendy; Hiroji Kanbara | Zendy

Open Access

POLYMORPHISM OF THE PLASMODIUM FALCIPARUM MULTIDRUG RESISTANCE AND CHLOROQUINE RESISTANCE TRANSPORTER GENES AND IN VITRO SUSCEPTIBILITY TO AMINOQUINOLINES IN ISOLATES FROM THE PERUVIAN AMAZON

Author(s) -

Maria Cecilia Huaman,

Norma Roncal,

Shusuke Nakazawa,

Ton That Ai Long,

Lucia Gerena,

Coralith García,

Lely Solari,

Alan J. Magill,

Hiroji Kanbara

Publication year - 2004

Publication title -

american journal of tropical medicine and hygiene

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.015

H-Index - 151

eISSN - 1476-1645

pISSN - 0002-9637

DOI - 10.4269/ajtmh.2004.70.461

Subject(s) - plasmodium falciparum , biology , mefloquine , chloroquine , virology , quinine , malaria , drug resistance , multiple drug resistance , apicomplexa , haplotype , gene , genetics , genotype , immunology

In vitro drug sensitivity to chloroquine (CQ), mefloquine (MQ) and quinine was investigated in 60 culture-adapted Plasmodium falciparum isolates from malaria patients in Padrecocha, a village in the Amazonian Department of Loreto, Peru. All isolates showed resistance to CQ, decreased susceptibility to quinine, and sensitivity to MQ. These isolates were examined for mutations in the P. falciparum multidrug resistance 1 (pfmdr1) and chloroquine resistance transporter (pfcrt) genes previously linked to CQ resistance. The mutations N86Y and D1246Y, two of the five mutations commonly observed in the pfmdr1 gene of CQ-resistant clones, were not found. The pfcrt mutation K76T, associated with CQ resistance, was identified in all the isolates tested. Sequence analysis of codons 72-76 in the pfcrt gene showed the haplotypes SVMNT and CVMNT.

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