Development of Three PCR Assays for the Differentiation between Echinococcus shiquicus, E. granulosus (G1 genotype), and E. multilocularis DNA in the Co-Endemic Region of Qinghai-Tibet plateau, China | Zendy

Belgees Boufana | Zendy; Gérald Umhang | Zendy; Jiamin Qiu | Zendy; Xingwang Chen | Zendy; Samia Lahmar | Zendy; Franck Boué | Zendy; David Jenkins | Zendy; Philip S. Craig | Zendy

Open Access

Development of Three PCR Assays for the Differentiation between Echinococcus shiquicus, E. granulosus (G1 genotype), and E. multilocularis DNA in the Co-Endemic Region of Qinghai-Tibet plateau, China

Author(s) -

Belgees Boufana,

Gérald Umhang,

Jiamin Qiu,

Xingwang Chen,

Samia Lahmar,

Franck Boué,

David Jenkins,

Philip S. Craig

Publication year - 2013

Publication title -

american journal of tropical medicine and hygiene

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.015

H-Index - 151

eISSN - 1476-1645

pISSN - 0002-9637

DOI - 10.4269/ajtmh.12-0331

Subject(s) - genotype , plateau (mathematics) , echinococcus multilocularis , echinococcus granulosus , biology , qinghai lake , dna , echinococcosis , gene , genetics , microbiology and biotechnology , zoology , mathematical analysis , paleontology , glacier , mathematics

To investigate echinococcosis in co-endemic regions, three polymerase chain reaction (PCR) assays based on the amplification of a fragment within the NADH dehydrogenase subunit 1 (ND1) mitochondrial gene were optimized for the detection of Echinococcus shiquicus, Echinococcus granulosus G1, and Echinococcus multilocularis DNA derived from parasite tissue or canid fecal samples. Specificity using parasite tissue-derived DNA was found to be 100% except for E. shiquicus primers that faintly detected E. equinus DNA. Sensitivity of the three assays for DNA detection was between 2 and 10 pg. Ethanol precipitation of negative PCR fecal samples was used to eliminate false negatives and served to increase sensitivity as exemplified by an increase in detection from 0% to 89% of E. shiquicus coproDNA using necropsy-positive fox samples.

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