Open AccessAssociation of variable number of tandem repeats polymorphism in the IL-4 gene with end-stage renal disease in Malaysian patients
Author(s) -
Vasudevan Ramachandran,
M.N. Norhasniza,
Ismail Patimah
Publication year - 2011
Publication title -
genetics and molecular research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.356
H-Index - 48
ISSN - 1676-5680
DOI - 10.4238/vol10-2gmr1066
Subject(s) - variable number tandem repeat , end stage renal disease , genotype , biology , agarose gel electrophoresis , genetics , genomic dna , gene , tandem repeat , allele , intron , disease , microbiology and biotechnology , medicine , genome
Variable number of tandem repeats (VNTR) polymorphism in the interleukin 4 (IL-4) gene has been associated with end-stage renal disease (ESRD) subjects in many different populations, although with conflicting results. We determined the 70 bp of VNTR polymorphism at intron 3 of the IL-4 gene in Malaysian ESRD subjects. Buccal cells were collected from 160 case and 160 control subjects; genomic DNA was amplified using PCR, followed by agarose gel electrophoresis. There were significant differences in genotypes and alleles of the IL-4 gene. We conclude that VNTR polymorphism of the IL-4 gene is a risk factor for the development of ESRD among Malaysians.
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