Adeno-associated virus-mediated BMP-7 and SOX9 in vitro co-transfection of human degenerative intervertebral disc cells | Zendy

Xianzhen Ren | Zendy; Z.Z. Diao | Zendy; Yongming Xi | Zendy; Zonghua Qi | Zendy; Shan Ren | Zendy; Y.J. Liu | Zendy; Dexin Yang | Zendy; X Zhang | Zendy; SiMing Yuan | Zendy

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Adeno-associated virus-mediated BMP-7 and SOX9 in vitro co-transfection of human degenerative intervertebral disc cells

Author(s) -

Xianzhen Ren,

Z.Z. Diao,

Yongming Xi,

Zonghua Qi,

Shan Ren,

Y.J. Liu,

Dexin Yang,

X Zhang,

SiMing Yuan

Publication year - 2015

Publication title -

genetics and molecular research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.356

H-Index - 48

ISSN - 1676-5680

DOI - 10.4238/2015.april.22.1

Subject(s) - transfection , sox9 , bone morphogenetic protein 2 , intervertebral disc , bone morphogenetic protein 7 , in vitro , microbiology and biotechnology , adeno associated virus , bone morphogenetic protein , chemistry , biology , gene expression , recombinant dna , anatomy , gene , biochemistry , vector (molecular biology)

Bone morphogenetic protein-7 (BMP-7) and SOX9 are important transcription factors in chondrogenesis. In this study, we examined the biological function of the adeno-associated virus (AAV)-mediated BMP-7 and SOX9 double gene in vitro co-transfection of nucleus pulposus cells of human degenerative intervertebral disc. Human intervertebral disc nucleus pulposus cells were cultured in vitro and subcultured for 5 generations. Using rAAV-BMP-7 and rAAV-SOX9 AAV2-type AAV viruses, the cells were divided into 4 groups: blank normal, BMP-7 transfection, SOX9 transfection, and BMP-7 and SOX9 co-transfection. After 48 h, expression of type II collagen and its mRNA in nucleus pulposus cells was determined. The expression of type II collagen in BMP-7 transfection, SOX9 transfection, and co-transfection groups was up-regulated to varying degrees compared to the blank control group. The type II collagen mRNA level expression in the co-transfection group was significantly higher than in other groups (P < 0.05). AAV-mediated BMP-7 and SOX9 in vitro co-transfection can promote the synthesis of type II collagen in nucleus pulposus cells in the human degenerative intervertebral disc. Double-gene therapy has a synergistic effect in the treatment of intervertebral disc degeneration.

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