Genetic diversity and relationships in cultivars of Lolium multiflorum Lam. using sequence-related amplified polymorphism markers | Zendy

Linkai Huang | Zendy; Xueyu Jiang | Zendy; Q.T. Huang | Zendy; Yang Xiao | Zendy; Zhongfa Chen | Zendy; X. Q. Zhang | Zendy; Jiamin Miao | Zendy; Hong Yan | Zendy

Open Access

Genetic diversity and relationships in cultivars of Lolium multiflorum Lam. using sequence-related amplified polymorphism markers

Author(s) -

Linkai Huang,

Xueyu Jiang,

Q.T. Huang,

Yang Xiao,

Zhongfa Chen,

X. Q. Zhang,

Jiamin Miao,

Hong Yan

Publication year - 2014

Publication title -

genetics and molecular research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.356

H-Index - 48

ISSN - 1676-5680

DOI - 10.4238/2014.december.4.8

Subject(s) - lolium multiflorum , biology , genetic diversity , germplasm , cultivar , genetic similarity , polymorphism (computer science) , lolium , genotype , genetics , botany , poaceae , population , gene , demography , sociology

Sequence-related amplified polymorphism (SRAP) markers were used to analyze and estimate the genetic variability, level of diversity, and relationships among 20 cultivars and strains of annual ryegrass (Lolium multiflorum Lam.). Eighteen SRAP primer combinations generated 334 amplification bands, of which 298 were polymorphic. The polymorphism information content ranged from 0.4715 (me10 + em1) to 0.5000 (me5 + em7), with an average of 0.4921. The genetic similarity coefficient ranged from 0.4304 to 0.8529, and coefficients between 0.65 and 0.90 accounted for 90.00%. The cluster analysis separated the accessions into five groups partly according to their germplasm resource origins.

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