Microarray profiles of ex vivo expanded hematopoietic stem cells show induction of genes involved in noncanonical Wnt signaling | Zendy

Dalila Lucíola Zanette | Zendy; Julio C. C. Lorenzi | Zendy; Rodrigo Alexandre Panepucci | Zendy; A. R. D. Santos | Zendy; Greice Andreotti de Molfetta | Zendy; Astolfo Gomes de Mello Araújo | Zendy; W.A. Silva | Zendy; Marco A. Zago | Zendy

Open Access

Microarray profiles of ex vivo expanded hematopoietic stem cells show induction of genes involved in noncanonical Wnt signaling

Author(s) -

Dalila Lucíola Zanette,

Julio C. C. Lorenzi,

Rodrigo Alexandre Panepucci,

A. R. D. Santos,

Greice Andreotti de Molfetta,

Astolfo Gomes de Mello Araújo,

W.A. Silva,

Marco A. Zago

Publication year - 2013

Publication title -

genetics and molecular research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.356

H-Index - 48

ISSN - 1676-5680

DOI - 10.4238/2013.may.15.1

Subject(s) - ex vivo , haematopoiesis , wnt signaling pathway , stem cell , biology , microbiology and biotechnology , in vivo , hematopoietic stem cell , microarray analysis techniques , microarray , immunology , signal transduction , gene , gene expression , genetics

The low number of hematopoietic stem cells (HSC) in umbilical cord blood (UCB) is directly related to increased risk of transplant failure. Effective ex vivo expansion of HSC has been tried for many years, with conflicting results because of the inability to reproduce in vitro HSC proliferation in the same way it occurs in vivo. We compared freshly isolated HSC with their expanded counterparts by microarray analysis and detected activation of the noncanonical Wnt (wingless-type MMTV integration site family) pathway. Study of early alterations during ex vivo UCB-HSC expansion could contribute to improvement of ex vivo expansion systems.

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