Review Genomic in situ hybridization in plants | Zendy

Gonçalo Santos Silva | Zendy; Margarete Magalhães Souza | Zendy

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Review Genomic in situ hybridization in plants

Author(s) -

Gonçalo Santos Silva,

Margarete Magalhães Souza

Publication year - 2013

Publication title -

genetics and molecular research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.356

H-Index - 48

ISSN - 1676-5680

DOI - 10.4238/2013.august.12.11

Subject(s) - biology , in situ hybridization , molecular cytogenetics , hybrid , genome , comparative genomic hybridization , meiosis , cytogenetics , fluorescence in situ hybridization , computational biology , in situ , genetics , chromosome , gene , gene expression , botany , physics , meteorology

Genomic in situ hybridization (GISH), which is a modification of fluorescent in situ hybridization, has been widely used in the study of plants. It has become one of the most important techniques for molecular cytogenetics. GISH is a technique that allows distinguishing the genomes in a cell. With this technique, it is possible to differentiate the genomes in a hybrid; consequently, this tool has been applied to the study of hybrid lineages, genetic improvement programs, and studies of the evolution of polyploids. Moreover, GISH can be applied to the analysis of the meiotic behavior in hybrids and polyploids, providing information concerning the relationship between species. This review presents the wide application of this technique in plants.

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