Development of genetic markers for Ochradenus arabicus (Resedaceae), an endemic medicinal plant of Saudi Arabia | Zendy

Salim Khan | Zendy; Fahad AlQurainy | Zendy; Mohammad Nadeem | Zendy; Mohamed Tarroum | Zendy

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Development of genetic markers for Ochradenus arabicus (Resedaceae), an endemic medicinal plant of Saudi Arabia

Author(s) -

Salim Khan,

Fahad AlQurainy,

Mohammad Nadeem,

Mohamed Tarroum

Publication year - 2012

Publication title -

genetics and molecular research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.356

H-Index - 48

ISSN - 1676-5680

DOI - 10.4238/2012.may.14.4

Subject(s) - internal transcribed spacer , biology , rpob , genetic marker , genus , chloroplast dna , endemism , identification (biology) , botany , ribosomal dna , dna sequencing , shrub , evolutionary biology , chloroplast , traditional medicine , genetics , ribosomal rna , dna , phylogenetics , gene , medicine , ecology , 16s ribosomal rna

Some species of the genus Ochradenus are difficult to identify based on morphological markers. Similar limitations are found for biochemical markers. We developed genetic markers based on DNA sequences for Ochradenus arabicus, which is an endemic plant to Saudi Arabia, locally utilized as a medicinal shrub. The internal transcribed spacer sequence of nuclear ribosomal DNA and chloroplast (rpoB and rpoC1) markers were more informative than other chloroplast DNA markers. Based on these markers, we were able to discriminate this species from another species of the same genus (O. baccatus) that is widely distributed in Saudi Arabia, despite a high degree of morphological similarity. These genetic markers facilitate its identification, even when acquired in a dried state from local markets.

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