Isolation and characterization of 25 microsatellite DNA loci for Anopheles albitarsis sensu lato and inter-specific amplification in 5 congeneric species | Zendy

Giselle Moura Guimarães-Marques | Zendy; Jacqueline da Silva Batista | Zendy; H.M. Guimarães | Zendy; M.V. Naice-Daou | Zendy; Maria Patelli Juliani Souza Lima | Zendy; Kyara Martins Formiga | Zendy; Joselita Maria Mendes dos Santos | Zendy; Carlos Alberto Praia Lima | Zendy; Míriam Silva Rafael | Zendy

Open Access

Isolation and characterization of 25 microsatellite DNA loci for Anopheles albitarsis sensu lato and inter-specific amplification in 5 congeneric species

Author(s) -

Giselle Moura Guimarães-Marques,

Jacqueline da Silva Batista,

H.M. Guimarães,

M.V. Naice-Daou,

Maria Patelli Juliani Souza Lima,

Kyara Martins Formiga,

Joselita Maria Mendes dos Santos,

Carlos Alberto Praia Lima,

Míriam Silva Rafael

Publication year - 2012

Publication title -

genetics and molecular research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.356

H-Index - 48

ISSN - 1676-5680

DOI - 10.4238/2012.december.6.3

Subject(s) - sensu , loss of heterozygosity , biology , microsatellite , genetics , population , population genetics , anopheles , allele , species complex , zoology , evolutionary biology , malaria , genus , gene , demography , sociology , immunology , phylogenetic tree

The Anopheles albitasis complex includes 6 species, and 3 are considered as malaria vectors in Brazil. Twenty-five polymorphic microsatellite DNA loci were isolated and characterized in 24-36 individuals from the neighborhood of Puraquequara, Manaus, Amazonas State, Brazil. The number of estimated alleles ranged from 2 to 10, the observed heterozygosity ranged from 0.182 to 0.897, and the expected heterozygosity ranged from 0.260 to 0.854. Eleven loci showed significant deviation from Hardy-Weinberg equilibrium. Eleven loci were cross-amplified successfully in 5 Anopheles species. These microsatellite loci will be useful in studies investigating population structure and evolutionary genetics in A. albitarsis sensu lato and other A. albitarsis complex species.

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