The Expression of CD8+ and MHC-I in Cervical Cancer with HPV Infection | Zendy

Adi Prayitno | Zendy; Elyana Asnar | Zendy; Okid Parama Astirin | Zendy; Suhartono Taat Putra | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

The Expression of CD8+ and MHC-I in Cervical Cancer with HPV Infection

Author(s) -

Adi Prayitno,

Elyana Asnar,

Okid Parama Astirin,

Suhartono Taat Putra

Publication year - 2013

Publication title -

journal of cancer therapy

Language(s) - English

Resource type - Journals

eISSN - 2151-1942

pISSN - 2151-1934

DOI - 10.4236/jct.2013.45a003

Subject(s) - cd8 , mhc class i , cytotoxic t cell , cervical cancer , major histocompatibility complex , immunology , cancer , medicine , immunity , biology , immune system , genetics , in vitro

Introduction: The immunity against cervical cancers is many unknown. Major histocompatability complex class I (MHC-I) with the alpha domain has binding site for Cytotoxic T cells (CD8+) to target cells. The objective of this experiment is to know the expression of CD8+ and MHC-I in cervical cancer with HPV infection. Method: DNA was isolated from seventeen sample cervical cancer tissues frozen section. Diagnose related with HPV was made by PCR. Paraffin block of the tissues was cut in thoroughly cleaned cryotome and place in glass plate that covered with poly-elysine. The immuno-histochemistry is done with monoclonal antibody again MHC-I and CD8+ with TSA-indirect method. The power of expression was counted the number of positive cells to express the MHC-I and CD8+ every 100 cells per one view look. Result: The Result of this experiment shows that expression of MHC-I and CD8+ in cervical cancer with HPV infection is in mild category (30% - 70%). Conclusion: Immunity against uterine cancer played by MHC-I and CD8+ is in milt category. The experiment that related with uterine cancer immunology is suggested.