In Silicon Cloning and Bioinformatics Analysis of the Raphanus Sativus WUS Gene | Zendy

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In Silicon Cloning and Bioinformatics Analysis of the Raphanus Sativus WUS Gene

Author(s) -

Guang Ma

Publication year - 2013

Publication title -

engineering

Language(s) - English

Resource type - Journals

eISSN - 1947-3931

pISSN - 1947-394X

DOI - 10.4236/eng.2013.510b104

Subject(s) - open reading frame , raphanus , cloning (programming) , in silico , gene , coding region , intron , complementary dna , computational biology , genetics , clone (java method) , biology , sequence analysis , bioinformatics , microbiology and biotechnology , peptide sequence , computer science , botany , programming language

The complete coding sequence ofRaphanus sativusWUS gene was obtained by usingArabidopsis thalianagene se-quence (NM_127349) as a probe in silico cloning. Then the hydrophilicity, secondary structure and advanced structure of WUS protein inRaphanus sativusare analyzed by using bioinformatics methods. The results show that the cDNA was 1298 bp, with no intron, contains an open reading frame of 936bp, encoding 312aa protein. The protein coded byRaphanus sativusgene showed 74% similarity toArabidopsis thaliana.

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