Therapeutic Potential of Bone Marrow Derived Mesenchymal Stem Cells in Modulating Astroglyosis of Surgical Induced Experimental Spinal Cord Injury | Zendy

Moataz A. Elawady | Zendy; Mohammed M. Elmaghrabi | Zendy; Nesrine Ebrahim | Zendy; Mona Ahmed Elawady | Zendy; Dina Sabry | Zendy; Ashraf Shamaa | Zendy; Alyaa Ragaei | Zendy

Open Access

Therapeutic Potential of Bone Marrow Derived Mesenchymal Stem Cells in Modulating Astroglyosis of Surgical Induced Experimental Spinal Cord Injury

Author(s) -

Moataz A. Elawady,

Mohammed M. Elmaghrabi,

Nesrine Ebrahim,

Mona Ahmed Elawady,

Dina Sabry,

Ashraf Shamaa,

Alyaa Ragaei

Publication year - 2016

Publication title -

advances in bioscience and biotechnology

Language(s) - English

Resource type - Journals

eISSN - 2156-8502

pISSN - 2156-8456

DOI - 10.4236/abb.2016.76024

Subject(s) - medicine , spinal cord injury , regeneration (biology) , spinal cord , mesenchymal stem cell , lesion , transplantation , glial scar , pathology , astrocyte , bone marrow , immunohistochemistry , central nervous system , surgery , biology , psychiatry , microbiology and biotechnology

Background: Spinal cord injury (SCI) unsuccessful regeneration was due to glial scar development. It was a major obstacle to axonal restoration. Safe therapeutic intervention by the use of bone marrow derived stem cells (BMMSCs) transplantation applied in the present study could reduce spinal disability. Material and methods: Forty male albino rats were divided into four groups: GI: negative control (n = 10 rats); GII: positive control after SCI (n = 10 rats); GIII: SCI + BM MSCs intravenous injected and GIV: SCI + BM MSCs intra lesion injected (n = 10 rats in each group). The samples were taken from spinal cord tissues around the region of injury and were subjected to histological, immunohistochemical assessment. RNA extraction and real time PCR for detection of nerve regeneration and astrocyte response to the injury were also performed. Results: Clinical improvement occurred by the enhancement in the Basso, Beattie and Bresnahan (BBB) score after SCI. Histological examinations showed positive regenerative responses in GIV compared to GIII. Conclusion: BM-MSCs transplantation has a promising role in enhancing the microenvironment for nerve regeneration through stumbling the glial scaring formation and inflammatory response after chronic spinal cord injury especially by using intra-lesion route injection.

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