Biological implications of 2-chlorocyclohexa-2,5-diene-1,4-dione toward ribonuclease A | Zendy

Albert R. Vaughn | Zendy; Caitlin B. Redman | Zendy; Sophia M. Kang | Zendy; Jisook Kim | Zendy

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Biological implications of 2-chlorocyclohexa-2,5-diene-1,4-dione toward ribonuclease A

Author(s) -

Albert R. Vaughn,

Caitlin B. Redman,

Sophia M. Kang,

Jisook Kim

Publication year - 2013

Publication title -

advances in bioscience and biotechnology

Language(s) - English

Resource type - Journals

eISSN - 2156-8502

pISSN - 2156-8456

DOI - 10.4236/abb.2013.41004

Subject(s) - ribonuclease , reactivity (psychology) , rnase p , chemistry , amino acid , serine , s tag , lysine , biochemistry , cysteine , ascorbic acid , stereochemistry , enzyme , rna , medicine , alternative medicine , food science , pathology , gene

2-Chlorocyclohexa-2,5-diene-1,4-dione (CBQ) or 2-chloro1,4-benzquinone is one of the common metabolites of polycyclic aromatic hydrocarbons generated through industrial processes. This report describes the biological effects of CBQ toward ribonuclease A (RNase). We also investigated the inhibition of RNase modifications and the reactivity of CBQ toward selected amino acids. The study was carried out by incubating RNase or amino acids with CBQ in a concentration- and a time-dependent manner at 37?C and pH 7.0. SDS-PAGE results showed oligomerization as well as polymeric aggregation of RNase when incubated with CBQ as early as in 10 min. CBQ-induced RNase modifications were inhibited in the presence of NADH or ascorbic acid. CBQ reactivity toward selected amino acids was also evaluated by determining the second-order rate constants for the reactions of CBQ with selected amino acids. It was found that the reactivity toward CBQ decreased in the order of lysine > threonine > serine >> aspartate > cysteine.

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