Fabrication of Gold Nanoparticles/Polypyrrole/HRP Electrode for Phenol Biosensor by Electropolymerization

A phenol enzyme biosensor was fabricated in this research using disposable screen-printed carbon electrode modified with gold nanoparticles (AuNPs), polypyrrole (PPy) and immobilized horseradish peroxidase (HRP) by electropolymerization method. The physical and chemical properties of the modified electrode was characterized by scanning electron microscopy (SEM), and energy dispersive X-ray spectroscopy (EDS) while electrochemical analysis was performed with amperometry. The experimental parameters were optimized with regard to the increasing concentration of AuO and enzyme amounts. The obtained result proved that AuNPs/Polypyrrole composite matrix could not only appropriately immobilize HRP, but also retain its bioactivity. Furthermore, the presence of gold nanoparticles provides enhanced electrochemical responses. The proposed method was simple, convenience to use, and illustrated high sensitivity.