Open AccessDirect Immunological Detection of Wood Cell Wall Polysaccharides after Microwave- Assisted Ionic Liquid Disruption
Author(s) -
Idelette Plazanet,
Rachida Zerrouki,
Sabine Lhernould,
Catherine Breton,
Guy Costa
Publication year - 2015
Publication title -
hal (le centre pour la communication scientifique directe)
Language(s) - English
DOI - 10.4172/2168-
Subject(s) - polysaccharide , ionic liquid , cell wall , microwave , chemistry , materials science , biochemistry , engineering , telecommunications , catalysis
We present here a new cell wall polysaccharide analysis method based on the fast and efficient ionic liquidsolubilization of wood samples followed by direct immunological detections of their polysaccharide contents. Toreduce both the time and the temperature of the analysis, wood powder samples are first totally disrupted in 1-ethyl-3-methylimidazolium under controlled microwave irradiation. The solubilized extracts are then directly spotted ontonitrocellulose membranes in order to specifically detect their polysaccharide contents with a set of monoclonal antibodies. Compared to the long sequential method classically used for biomass analysis, the technique described in this article allows the simultaneous treatments of higher numbers of smaller samples. It represents an interesting toolkit to rapidly screen various biomass resources for their polysaccharide contents