Open AccessMolecular Epidemiology ofClostridium perfringensIsolated from Food Poisoning in Seoul, 2013
Author(s) -
Young-Hee Jin,
Jihun Jung,
Su-Jin Jeon,
Seong-Seon Choi,
Youngeun Kim,
YoungHee Oh,
Sung-Min Choi,
Kweon Jung
Publication year - 2014
Publication title -
journal of bacteriology and virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.179
H-Index - 12
eISSN - 2093-0429
pISSN - 1598-2467
DOI - 10.4167/jbv.2014.44.2.170
Subject(s) - pulsed field gel electrophoresis , clostridium perfringens , food poisoning , microbiology and biotechnology , molecular epidemiology , outbreak , biology , toxin , epidemiology , gel electrophoresis , asymptomatic carrier , gene , genotype , virology , asymptomatic , medicine , bacteria , genetics , pathology
Clostridium perfringens food poisoning ranks among the most common gastrointestinal diseases in developed countries. In Korea, C. perfringens food poisoning gradually increases. Using PCR, 72 strains of C. perfringens isolated in Seoul, 2013 were tested for the presence of toxin genes. Of the tested strains, 32 isolates carried the cpe gene, 37 isolates carried the cpb2 gene and 3 isolates carried the cpe and cpb2 genes, respectively. 32 cpe- positive strains were isolated from the food poisoning patient, whereas among 37 cpb2-positive strains, 22 strains were isolated from asymptomatic person. To investigate epidemiological relationship between the isolates, Pulsed-filed gel electrophoresis (PFGE) was performed. The genetic relatedness of the isolates ranged from 55.9% to 100% and 47 distinct PFGE profiles were observed. The results show that the cpe- positive outbreak strains showed close genetic relation, whereas the cpb2-positive isolates revealed a wide genetic diversity.
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