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Aim:  To develop a useful alternative approach to evaluate the gene function in hairy roots.    Methods:   Arabidopsis  and tobacco (wild-type or mutant) were a host for  Agrobacterium rhizogenes  transformation.    Results:  The hairy roots formation efficiency ranged from 53 to 98% in tobacco and 53 to 66% in  Arabidopsis . Hairy and intact roots showed similar gene expression pattern in response to salt and aluminum stress. Genomic polymerase chain reaction and fluorescent images showed high rate (>80%) of co-integration of T-DNAs and uniform cell transformation without use of any antibiotic selection. Whole processes of hairy roots were completed within 1 month after the infection of  Agrobacterium .    Conclusion:  Aluminum-responsive orthologous gene function could be evaluated by  NtSTOP1- KD and  Atstop1  as a host for hairy roots transformation.

Agrobacterium rhizogenes-mediated hairy roots transformation as a tool for exploring aluminum-responsive genes function