Open AccessMaintenance of Head and Neck Tumor On-Chip: Gateway to Personalized Treatment?
Author(s) -
Ruth Bower,
Victoria L. Green,
Elena Kuvshinova,
Dmitriy Kuvshinov,
Laszlo Karsai,
Stephen Crank,
Nicholas D. Stafford,
John Greenman
Publication year - 2017
Publication title -
future science oa
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.825
H-Index - 23
ISSN - 2056-5623
DOI - 10.4155/fsoa-2016-0089
Subject(s) - propidium iodide , flow cytometry , trypan blue , head and neck squamous cell carcinoma , cancer research , microfluidics , head and neck cancer , pathology , medicine , chemistry , cancer , biology , cell , apoptosis , microbiology and biotechnology , programmed cell death , materials science , nanotechnology , biochemistry
Aim: Head and neck squamous cell carcinomas (HNSCC) are solid tumors with low overall survival (40–60%). In a move toward personalized medicine, maintenance of tumor biopsies in microfluidic tissue culture devices is being developed. Methodology/results: HNSCC (n = 15) was dissected (5–10 mg) and either analyzed immediately or cultured in a microfluidic device (37°C) for 48 h. No difference was observed in morphology between pre- and postculture specimens. Dissociated samples were analyzed using trypan blue exclusion (viability), propidium iodide flow cytometry (death) and MTS assay (proliferation) with no significant difference observed highlighting tissue maintenance. Computational fluid dynamics showed laminar flow within the system. Conclusion: The microfluidic culture system successfully maintained HNSCC for 48 h, the culture system will allow testing of different treatment modalities with response monitoring.
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