Open AccessAn integrated versatile lab-on-a-chip platform for the isolation and nucleic acid-based detection of pathogens
Author(s) -
Natalia Sandetskaya,
Doreen Moos,
Harald Pötter,
Stefan Seifert,
Marcin Jenerowicz,
Holger Becker,
Christian Zilch,
Dirk Kuhlmeier
Publication year - 2017
Publication title -
future science oa
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.825
H-Index - 23
ISSN - 2056-5623
DOI - 10.4155/fsoa-2016-0088
Subject(s) - loop mediated isothermal amplification , nucleic acid , microfluidics , dna extraction , lysis , isolation (microbiology) , microfluidic chip , computer science , computational biology , dna , sample preparation , nucleic acid quantitation , molecular diagnostics , nucleic acid methods , polymerase chain reaction , nanotechnology , chemistry , biology , chromatography , microbiology and biotechnology , bioinformatics , materials science , biochemistry , gene
Aim: Processing of the samples in molecular diagnostics is complex and labor-intensive. An integrated and automated platform for sample preparation and nucleic acid-based detection can significantly relieve this burden for the users. Results: We present a prototype of a versatile and integrated platform for the detection of pathogens in various liquid media. We describe a proof-of-concept for the integrated isolation of bacteria, cell lysis with optional DNA extraction, DNA amplification and detection in two different reactions, loop-mediated isothermal amplification and PCR, on a single microfluidic platform. Conclusion: The platform enables the transition from large sample volume to microfluidic format. The design and open interface enable its versatile application for various nucleic acid-based assays, from simple to complex setups.
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