Novel automated immune turbidimetric assay for routine urinary cystatin-C determinations | Zendy

Balázs Szirmay | Zendy; Péter Kustán | Zendy; Zoltán HorváthSzalai | Zendy; Andrea Ludány | Zendy; Ágnes Lakatos | Zendy; Diána Mühl | Zendy; István Wittmann | Zendy; Attila Miseta | Zendy; Gábor L. Kovaćs | Zendy; Tamás Kőszegi | Zendy

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Novel automated immune turbidimetric assay for routine urinary cystatin-C determinations

Author(s) -

Balázs Szirmay,

Péter Kustán,

Zoltán HorváthSzalai,

Andrea Ludány,

Ágnes Lakatos,

Diána Mühl,

István Wittmann,

Attila Miseta,

Gábor L. Kovaćs,

Tamás Kőszegi

Publication year - 2018

Publication title -

bioanalysis

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.566

H-Index - 58

eISSN - 1757-6199

pISSN - 1757-6180

DOI - 10.4155/bio-2017-0228

Subject(s) - cystatin c , turbidimetry , urinary system , chromatography , immune system , chemistry , microchemistry , cystatin , medicine , immunology , biochemistry , creatinine

Aim: There is no commercially available urinary cystatin-C (u-CYSC) test in the market. Therefore, we optimized and validated an automated immune turbidimetric test for u-CYSC measurements and investigated u-CYSC concentrations in acute and chronic diseases which might lead to renal tubular disorders. Materials & methods: A particle-enhanced immune turbidimetric assay was adapted and validated on a Cobas 8000/c502 analyzer. Urine samples of different patient groups were also analyzed. Results: Our method showed excellent analytical performance. U-CYSC/u-creatinine (u-CREAT) was higher in sepsis-related acute kidney injury group (p < 0.001) compared with controls and to patients with chronic hypertension and Type 2 diabetes. Conclusion: We validated a fast, sensitive, fully automated u-CYSC assay which is ideal for routine use and might be a potential complementary laboratory test to evaluate renal tubular function.

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