Electrochemical Synthesis of Silver Nanoparticles and their Potential Use as Antimicrobial Agent: a Case Study on Escherichia Coli
Author(s) -
Lucas Blandón,
Mario V. Vázquez,
Dora Benjumea,
Gelmy Ciro
Publication year - 2012
Publication title -
portugaliae electrochimica acta
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.252
H-Index - 27
eISSN - 1647-1571
pISSN - 0872-1904
DOI - 10.4152/pea.201202135
Subject(s) - antimicrobial , escherichia coli , electrochemistry , silver nanoparticle , combinatorial chemistry , nanoparticle , nanotechnology , chemistry , nuclear chemistry , materials science , biochemistry , organic chemistry , electrode , gene
Silver nanoparticles (AgNP) were synthesized by electrochemical method using high purity metallic silver in the presence of the surfactant Pluronic® f127. The synthesized nanoparticles were characterized using UV-Vis spectroscopy, Dynamic Light Scattering (DLS), X-Ray Dispersion Spectroscopy (XDS) and Scanning Electron Microscopy (SEM). The antimicrobial activity of AgNP was evaluated against Escherichia Coli. In presence of surfactant, the prepared nanoparticles led to a monomodal distribution with an average size less than 100 nm. The minimal inhibitory concentration (MIC) of the resulting suspension was evaluated using micro dilution method and the MIC results are satisfactory .
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