Isolation of cholesterol-lowering lactic acid bacteria from human intestine for probiotic use | Zendy

Hyeong Jun Lim | Zendy; So Young Kim | Zendy; Wan Kyu Lee | Zendy

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Isolation of cholesterol-lowering lactic acid bacteria from human intestine for probiotic use

Author(s) -

Hyeong Jun Lim,

So Young Kim,

Wan Kyu Lee

Publication year - 2004

Publication title -

journal of veterinary science

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.547

H-Index - 47

eISSN - 1976-555X

pISSN - 1229-845X

DOI - 10.4142/jvs.2004.5.4.391

Subject(s) - probiotic , bifidobacterium , lactobacillus , lactic acid , microbiology and biotechnology , cholesterol , bacteria , biology , bile acid , streptococcus , food science , chemistry , biochemistry , fermentation , genetics

Cholesterol-lowering effect of lactic acid bacteria (LAB: Streptococcus, Lactobacillus and Bifidobacterium) is well-known. Thus, we investigated LAB isolated from human intestine on the cholesterol-lowering effect in vitro. Seven Streptococcus (61.1%), 11 Lactobacillus (71.8%) and 7 Bifidobacterium (27.9%) were isolated as acid (pH 2.5 and 3.0) and bile (0.3% oxgall) tolerant strains. Streptococcus HJS-1, Lactobacillus HJL-37 and Bifidobacterium HJB-4 were finally selected as probiotic strains to use through the bile salt hydrolase (BSH) activity assay by using MRS media added taurodeoxycholic acid (TDCA) and the cholesterol-lowering test by using soluble cholesterol containing MRS broth. These studies suggested that the isolated LAB had an excellent hypocholesterolemic effect.

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