Studies of cocktail therapy with multiple cytokines for neoplasia or infectious disease of the dog I. cDNA cloning of canine IL-3 and IL-6 | Zendy

InSoo Shin | Zendy; H R Kim | Zendy; Myeong Jin Nam | Zendy; H Y Youn | Zendy

Open Access

Studies of cocktail therapy with multiple cytokines for neoplasia or infectious disease of the dog I. cDNA cloning of canine IL-3 and IL-6

Author(s) -

InSoo Shin,

H R Kim,

Myeong Jin Nam,

H Y Youn

Publication year - 2001

Publication title -

journal of veterinary science

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.547

H-Index - 47

eISSN - 1976-555X

pISSN - 1229-845X

DOI - 10.4142/jvs.2001.2.2.115

Subject(s) - complementary dna , genbank , microbiology and biotechnology , biology , open reading frame , peptide sequence , amino acid , coding region , rapid amplification of cdna ends , signal peptide , nucleic acid sequence , gene , biochemistry

This paper describes the cloning and sequence analysis of the cDNAs encoding the canine homologues of interleukin-3 (IL-3) and interleukin-6 (IL-6). The coding sequences for canine IL-3 and IL-6 were obtained by using the reverse transcription polymerase chain reaction (RT-PCR) with RNA harvested from canine peripheral blood mononuclear cells (PBMCs). Canine IL-3 cDNA includes a single open reading frame of 432 nucleotides, which encodes a 143 amino acid polypeptide and has 44.7, 42.4, 37 and 23.7% homology with the cow, sheep, human and rat IL-3 sequences, respectively. Canine IL-6 cDNA (GenBank accession number; AF275796) encodes a putative 20-amino acid signal peptide followed by a 187-amino acid mature protein. The predicted amino acid sequence of canine IL-6 shares 60.4, 77.2, 71.0, 55.8 and 42.0% sequence identity with those of human, feline, porcine, sheep and rat IL-6, respectively.

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