Detection of Lawsonia intracellularis in diagnostic specimens by one-step PCR | Zendy

Dong Kyun Suh | Zendy; Suk Kyung Lym | Zendy; You-Chan Bae | Zendy; Keun Woo Lee | Zendy; Won Pil Choi | Zendy; Jae Chan Song | Zendy

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Detection of Lawsonia intracellularis in diagnostic specimens by one-step PCR

Author(s) -

Dong Kyun Suh,

Suk Kyung Lym,

You-Chan Bae,

Keun Woo Lee,

Won Pil Choi,

Jae Chan Song

Publication year - 2000

Publication title -

journal of veterinary science

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.547

H-Index - 47

eISSN - 1976-555X

pISSN - 1229-845X

DOI - 10.4142/jvs.2000.1.1.33

Subject(s) - lawsonia intracellularis , polymerase chain reaction , feces , biology , enteritis , microbiology and biotechnology , virology , clone (java method) , genomic dna , dna , gene , genetics

Lawsonia intracellularis is not culturable with a standard bacteriologic culture. One step PCR assay as a clinical diagnostic method was developed for the rapid detection of porcine proliferative enteritis (PPE) caused by L. intracellularis. Primers were designed based on the p78 DNA clone of L. intracellularis. The one step PCR resulted in the formation of a specific 210-bp DNA product derived from L. intracellularis. The nonspecific amplification product was not detected with swine genomic DNA or other bacterial strains causing similar symptoms to L. intracellularis infection. The one step PCR was as sensitive as 100 pg of L. intracellularis genomic DNA. We applied this method to field specimens diagnosed as PPE by macroscopic observation. Of 17 mucosal scraping specimens, 16 (94%) were identified as positive to PPE and 15 (88%) of 17 feces specimens. These results suggest that the one step PCR can be used as a rapid diagnostic method for L. intracellularis infection.

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