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Sap flow gauges using a heat balance have been reliable for measuring real-time transpiration in a number of crops. However, information on the accuracy of sap flow gauges in canola is lacking. Therefore, a study was conducted to validate the sap flow system in canola and to observe sap flow response to variations in temperature and solar radiation. There were strong relationships between sap flow measured with sap flow gauges and actual transpiration measured by the gravimetric method over short periods of 1 h (r 2  = 0.93 and RMSE = 2.34 g h -1 ), and over longer periods of 1 d (r 2  = 0.83 and RMSE = 48 g d -1 ), although sap flow slightly overestimated transpiration. In both cases the slope was not significantly different from 1. Water use in canola, estimated with sap flow gauges or from actual transpiration measurement, was dependent upon temperature (r 2  = 0.94 to 0.96). Water use increased until daytime temperatures reached 36°C, after which water use decreased. Sap flow followed solar radiation trends in the field. Heat is lost or dissipated from the gauges convectively as the sap flows through the stem, conductively through the solid stem material, and radially into the surrounding air. As the convective proportion of the heat loss from the gauge increased, the accuracy of the water use estimation using the sap flow gauges increased. For sunny days, convective heat loss through sap flow accounted for a major portion of the total heat input to the gauges, while on cloudy days radial heat loss from the gauges accounted for a major portion of the heat input. Thus, at low sap flow rates during cloudy days, the possibility of error in the sap flow system was high. Overall, sap flow in canola was strongly related to daily solar radiation (r 2  = 0.92). The sensitive response to weather variations and the possibility of improving the accuracy at high flow rates in the field makes the use of sap flow gauges a viable option for measuring real-time transpiration in canola. Key words: Brassica napus, canola, heat balance, sap flow, transpiration, temperature, solar radiation

Determination of the water use and water use response of canola to solar radiation and temperature by using heat balance stem flow gauges