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The effect of five different glycerol equilibration times at 5 °C on survival of frozen-thawed sperm was evaluated in semen samples from 18 bulls. Sperm motility in frozen-thawed semen samples increased progressively with increase in equilibration time up to 6 h. There was no significant difference in sperm survival when equilibrated for 18 h compared to that equilibrated for 6 h.

EFFECT OF GLYCEROL EQUILIBRATION TIME ON SURVIVAL OF BULL SPERM FROZEN IN PELLETS