Lactation induces upregulation of the ubiquitin-mediated proteolytic pathway in skeletal muscle of dairy cows but does not alter hepatic expression | Zendy

Susan L. Greenwood | Zendy; Thomas C. Wright | Zendy; N.G. Purdie | Zendy; John Doelman | Zendy; J.P. Cant | Zendy; Brian W. McBride | Zendy

Open Access

Lactation induces upregulation of the ubiquitin-mediated proteolytic pathway in skeletal muscle of dairy cows but does not alter hepatic expression

Author(s) -

Susan L. Greenwood,

Thomas C. Wright,

N.G. Purdie,

John Doelman,

J.P. Cant,

Brian W. McBride

Publication year - 2009

Publication title -

canadian journal of animal science

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.377

H-Index - 64

eISSN - 1918-1825

pISSN - 0008-3984

DOI - 10.4141/cjas08125

Subject(s) - downregulation and upregulation , skeletal muscle , lactation , ubiquitin , proteolysis , messenger rna , endocrinology , medicine , protein degradation , chemistry , biology , biochemistry , enzyme , pregnancy , gene , genetics

The current study investigates regulation of mRNA expression of components of ubiquitin-mediated proteolysis in transition dairy cows. Longissimus dorsi muscle (exp. 1) and liver (exp. 2) biopsies were collected from Holstein dairy cows at 27 and 16 d pre-partum, respectively, and 3 and 10 d post-partum, respectively. Regulation of C8, E2, and ubiquitin mRNA expression was determined. Upregulation of skeletal muscle C8 (P = 0.09) and ubiquitin (P = 0.004) mRNA expression occurred post-partum compared with pre-partum. No regulation of hepatic mRNA expression was observed. In conclusion, ubiquitin-mediated proteolysis may contribute to skeletal muscle protein degradation during the periparturient period, and could provide a potential mechanism for attenuation of body protein loss at the onset of lactation.Key words: Lactation, ubiquitin-mediated proteolytic pathway, muscle (skeletal), liver

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