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In the dissolution-dynamic nuclear polarization technique, molecular probes with long T 1s are preferred. 13C nuclei of small molecules with no directly bonded protons or sp(3 13)C nuclei with proton positions substituted by deuterons may fulfill this requirement. The T 1 determination of such new molecular probes is crucial for the success of the hyperpolarized observation. Although the inversion-recovery approach remained by and large the standard for T 1 measurements, we show here that the steady-state variable nutation angle approach is faster and may be better suited for the determination of relatively long T 1s in thermal equilibrium. Specifically, the T 1 of a new molecular probe, [uniformly labeled (UL)-13C6, UL-2H8]2-deoxy-d-glucose, is determined here and compared to that of [UL-13C6, UL-2H7]d-glucose.

magnetic resonance insights

Application of the Steady-state Variable Nutation Angle Method for Faster Determinations of Long <i>T</i><sub>1</sub>S–-an Approach Useful for the Design of Hyperpolarized Mr Molecular Probes

Application of the Steady-state Variable Nutation Angle Method for Faster Determinations of Long T1S–-an Approach Useful for the Design of Hyperpolarized Mr Molecular Probes

Application of the Steady-state Variable Nutation Angle Method for Faster Determinations of Long T₁S–-an Approach Useful for the Design of Hyperpolarized Mr Molecular Probes