Open AccessEnumeration of Organohalide Respirers in Municipal Wastewater Anaerobic Digesters
Author(s) -
Bryan J.K. Smith,
Melissa Anne Boothe,
Brice A. Fiddler,
Tania M. Lozano,
Russel K. Rahi,
Mark J. Krzmarzick
Publication year - 2015
Publication title -
microbiology insights
Language(s) - English
Resource type - Journals
ISSN - 1178-6361
DOI - 10.4137/mbi.s31445
Subject(s) - enumeration , wastewater , anaerobic exercise , environmental science , chemistry , waste management , microbiology and biotechnology , pulp and paper industry , mathematics , biology , environmental engineering , engineering , combinatorics , physiology
Organohalide contaminants such as triclosan and triclocarban have been well documented in municipal wastewater treatment plants (WWTPs), but the degradation of these contaminants is not well understood. One possible removal mechanism is organohalide respiration by which bacteria reduce the halogenated compound. The purpose of this study was to determine the abundance of organohalide-respiring bacteria in eight WWTP anaerobic digesters. The obligate organohalide respiring Dehalococcoides mccartyi was the most abundant and averaged 3.3 × 10(7) copies of 16S rRNA genes per gram, while the Dehalobacter was much lower at 2.6 × 10(4) copies of 16S rRNA genes per gram. The genus Sulfurospirillum spp. was also detected at 1.0 × 10(7) copies of 16S rRNA genes per gram. No other known or putatively organohalide-respiring strains in the Dehalococcoidaceae family were found to be present nor were the genera Desulfitobacterium or Desulfomonile.
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