Determination of L-tryptophan and L-kynurenine in Human Serum by using LC-MS after Derivatization with (R)-DBD-PyNCS | Zendy

Hayato Ohashi | Zendy; Hideaki Iizuka | Zendy; Shunsuke Yoshihara | Zendy; Hayato Otani | Zendy; Misato Kume | Zendy; Kiyomi Sadamoto | Zendy; Hideaki Ichiba | Zendy; Takeshi Fukushima | Zendy

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Determination of L-tryptophan and L-kynurenine in Human Serum by using LC-MS after Derivatization with (R)-DBD-PyNCS

Author(s) -

Hayato Ohashi,

Hideaki Iizuka,

Shunsuke Yoshihara,

Hayato Otani,

Misato Kume,

Kiyomi Sadamoto,

Hideaki Ichiba,

Takeshi Fukushima

Publication year - 2013

Publication title -

international journal of tryptophan research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.405

H-Index - 23

ISSN - 1178-6469

DOI - 10.4137/ijtr.s11459

Subject(s) - kynurenine , tryptophan , derivatization , metabolite , chemistry , chromatography , liquid chromatography–mass spectrometry , serum concentration , mass spectrometry , endocrinology , biochemistry , amino acid , biology

Concentrations of l-tryptophan (l-Trp) and its metabolite, l-kynurenine (l-KYN), in sera of 19 normal subjects (age: 23.6 ± 3.5 y, male: 8, female: 11) were determined by high-performance liquid chromatography with mass-spectrometric detection, following their derivatization with (R)-(-)-4-(N, N-dimethylaminosulfonyl)-7-(3-isothiocyanatopyrrolidin-1-yl)-2,1,3-benzoxadiazole (DBD-PyNCS). A significant positive correlation between l-Trp and l-KYN concentrations was observed (r = 0.532, P < 0.05). Serum l-Trp concentration in male subjects (95.65 ± 4.27 μM) was significantly higher than that in female subjects (79.20 ± 3.34 μM; P < 0.05), while no significant differences in l-KYN concentration or the l-KYN:l-Trp ratio were observed between male and female subjects.

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