H HRMAS NMR Derived Bio-markers Related to Tumor Grade, Tumor Cell Fraction, and Cell Proliferation in Prostate Tissue Samples | Zendy

Katarina Stenman | Zendy; Pär Stattin | Zendy; Hans Stenlund | Zendy; Katrine Riklund | Zendy; Gerhard Gröbner | Zendy; Anders Bergh | Zendy

Open Access

H HRMAS NMR Derived Bio-markers Related to Tumor Grade, Tumor Cell Fraction, and Cell Proliferation in Prostate Tissue Samples

Author(s) -

Katarina Stenman,

Pär Stattin,

Hans Stenlund,

Katrine Riklund,

Gerhard Gröbner,

Anders Bergh

Publication year - 2011

Publication title -

biomarker insights

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.075

H-Index - 31

ISSN - 1177-2719

DOI - 10.4137/bmi.s6794

Subject(s) - creatine , prostate cancer , magic angle spinning , prostate , pathology , choline , medicine , chemistry , nuclear magnetic resonance spectroscopy , cancer , organic chemistry

A high-resolution magic angle spinning NMR spectroscopic approach is presented for evaluating the occurrence, amount and aggressiveness of cancer in human prostate tissue samples. Using this technique, key metabolites in malignant and non-malignant samples (n = 149) were identified, and patterns of their relative abundance were analyzed by multivariate statistical methods. Ratios of various metabolites - including (glycerophophorylcholine + phosphorylcholine)/creatine, myo-inositol/scyllo-inositol, scyllo-inositol/creatine, choline/creatine, and citrate/creatine - correlated with: i) for non-malignant tissue samples, the distance to the nearest tumor and its Gleason score and; ii) the fraction of tumor cells present in the sample; and iii) tumor cell proliferation (Ki67 labelling index). This NMR-based approach allows the extraction of information that could be useful for developing novel diagnostic methods for prostate cancer.

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