Production of (2R,3S)-2-Benzamidomethyl-3-Hydroxybutanoates by Immobilized Plant Cells of Parthenocissus Tricuspidata | Zendy

Kei Shimoda | Zendy; Naoji Kubota | Zendy; Hatsuyuki Hamada | Zendy; Tatsunari Kobayashi | Zendy; Hiroki Hamada | Zendy; Siddiqi Muhammad Shafi | Zendy; Nobuyoshi Nakajima | Zendy

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Production of (2R,3S)-2-Benzamidomethyl-3-Hydroxybutanoates by Immobilized Plant Cells of Parthenocissus Tricuspidata

Author(s) -

Kei Shimoda,

Naoji Kubota,

Hatsuyuki Hamada,

Tatsunari Kobayashi,

Hiroki Hamada,

Siddiqi Muhammad Shafi,

Nobuyoshi Nakajima

Publication year - 2009

Publication title -

biochemistry insights

Language(s) - English

Resource type - Journals

ISSN - 1178-6264

DOI - 10.4137/bci.s961

Subject(s) - yield (engineering) , incubation , calcium alginate , chemistry , horticulture , calcium , biology , biochemistry , materials science , organic chemistry , metallurgy

Incubation of methyl 2-benzamidomethyl-3-oxobutanoate with cultured plant cells of Parthenocissus tricuspidata for 2 days afforded methyl (2 R ,3 S )-2-benzamidomethyl-3-hydroxybutanoate with 100% de and >99% ee in 51% yield. The use of immobilized cells of P. tricuspidata in calcium alginate gel improved the yield of the product (95% yield) with 100% de and >99% ee. The immobilized cells of P. tricuspidata maintained the high potential for the reduction of methyl 2-benzamidomethyl-3-oxobutanoate (85% yield) after 5 times of usage

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